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IDCIA: Immunocytochemistry Dataset for Cellular Image Analysis

Abdurahman Ali Mohammed, Catherine Fonder, Donald S. Sakaguchi, Wallapak Tavanapong, Surya K. Mallapragada, Azeez Idris

TL;DR

A new annotated microscopic cellular image dataset to improve the effectiveness of machine learning methods for cellular image analysis, and indicates that none of the five existing models under this study are able to achieve sufficiently accurate count to replace the manual methods.

Abstract

We present a new annotated microscopic cellular image dataset to improve the effectiveness of machine learning methods for cellular image analysis. Cell counting is an important step in cell analysis. Typically, domain experts manually count cells in a microscopic image. Automated cell counting can potentially eliminate this tedious, time-consuming process. However, a good, labeled dataset is required for training an accurate machine learning model. Our dataset includes microscopic images of cells, and for each image, the cell count and the location of individual cells. The data were collected as part of an ongoing study investigating the potential of electrical stimulation to modulate stem cell differentiation and possible applications for neural repair. Compared to existing publicly available datasets, our dataset has more images of cells stained with more variety of antibodies (protein components of immune responses against invaders) typically used for cell analysis. The experimental results on this dataset indicate that none of the five existing models under this study are able to achieve sufficiently accurate count to replace the manual methods. The dataset is available at https://figshare.com/articles/dataset/Dataset/21970604.

IDCIA: Immunocytochemistry Dataset for Cellular Image Analysis

TL;DR

A new annotated microscopic cellular image dataset to improve the effectiveness of machine learning methods for cellular image analysis, and indicates that none of the five existing models under this study are able to achieve sufficiently accurate count to replace the manual methods.

Abstract

We present a new annotated microscopic cellular image dataset to improve the effectiveness of machine learning methods for cellular image analysis. Cell counting is an important step in cell analysis. Typically, domain experts manually count cells in a microscopic image. Automated cell counting can potentially eliminate this tedious, time-consuming process. However, a good, labeled dataset is required for training an accurate machine learning model. Our dataset includes microscopic images of cells, and for each image, the cell count and the location of individual cells. The data were collected as part of an ongoing study investigating the potential of electrical stimulation to modulate stem cell differentiation and possible applications for neural repair. Compared to existing publicly available datasets, our dataset has more images of cells stained with more variety of antibodies (protein components of immune responses against invaders) typically used for cell analysis. The experimental results on this dataset indicate that none of the five existing models under this study are able to achieve sufficiently accurate count to replace the manual methods. The dataset is available at https://figshare.com/articles/dataset/Dataset/21970604.

Paper Structure

This paper contains 9 sections, 3 equations, 3 figures, 6 tables.

Figures (3)

  • Figure 1: Scaffold for cells undergoing an electrical stimulation. The red box indicates the cell culture region.
  • Figure 2: Quantification of immature neurons in AHPCs after 7 days in vitro (DIV) of electrical stimulation. Row 1 (A-C) shows fluorescence images of AHPCs labeled with an immature neuron marker (TuJ1, red; A and C) and nuclei marker (DAPI, blue; B and C) following 15 min. of 125 mV electrical stimulation once a day for 7 days. Row 2 (D-E) shows the dot-annotated images of the TuJ1 (D) and DAPI (E) staining by using the ImageJ Cell Counter tool to put a pink dot on a cell to be counted. Scale bar = 50 µm. Images have been pseudo-colorized for better visualization.
  • Figure 3: Images (left) and generated density maps (right)